I-Pepstatin iyi-pentapeptide, i-aspartyl protease inhibitor eyenzeka ngokwemvelo, engavimbela i-aspartic protease kanye ne-acid protease yama-microorganisms ahlukahlukene.I-Pepstatin ikhiqizwa ikakhulukazi yizinhlobo ze-Streptomyces futhi ikhiqizwa yi-Streptomyces.Ingavimbela i-pepsin, i-pepsin D ne-angiotensin-releasing enzyme, futhi inemiphumela yokwelapha esilonda esiswini, umfutho wegazi ophakeme wezinso, isifo samathambo, i-carrageenan edema nezinye izifo.
I-Pepstatin iyi-inhibitor eqinile ye-aspartyl proteases njenge-pepsin, i-cathepsin D, ne-renin.Le pentapeptide yemvelo ehlukanisiwe ne-actinomycetes bekuyiminyaka eminingi i-renin inhibitor yakudala ku-vitro.I-Pepstatin ayiqondile ku-renin futhi ayincibiliki kahle emanzini.Okuphuma kusakhiwo se-pepstatin kukhuphule ukuncibilika kwayo kanye nokucaciswa kwayo kwe-renin ngama-oda amaningana wobukhulu.I-Pepstatin iqukethe i-γ amino acid statin engajwayelekile engase ithathele indawo yama-amino acid amabili kusibopho esiyisikelo se-protein substrate futhi ivimbe ukuqhekeka kwe-substrate ngenxa yokufana kwesakhiwo. esimweni soguquko se-peptide bond hydrolysis ngama-aspartyl proteases.
Lapho amaprotheni ekhishwa emangqamuzaneni aphukile, ama-protease angakhululwa, okudingeka avinjwe ngokushesha ukuze amaprotheni angonakali.Enqubweni yokukhishwa kwamaprotheni, ama-protease inhibitors adinga ukungezwa ukuvimbela i-proteolysis.I-Protease inhibitor ngokubanzi ibhekisela entweni ebophezela kwamanye amaqembu esikhungweni esisebenzayo sama-molecule e-protease, ukuze umsebenzi we-protease wehle noma unyamalale, kodwa ungashintshi iphrotheni ye-enzyme.Ukuzwela kwama-protease ahlukahlukene kumaprotheni ahlukene kuhlukile, ngakho-ke kuyadingeka ukulungisa ukuhlushwa kwama-protease ahlukahlukene.Ngenxa yokuthi ukunyibilika kwe-protease inhibitor oketshezini kuphansi kakhulu, kufanele kunakwe ngokukhethekile ukuthi i-protease inhibitor kufanele ixutshwe ngokugcwele ku-buffer ukuze kwehliswe ukuna kwe-protease inhibitor.I-Pepstantin A ingavimbela ama-acid proteases njenge-pepsin, i-angiotensin, i-collagenase, i-cathepsin D ne-chymosin.
I-Pepstatin A iyi-inhibitor ye-cathepsin d ne-e.Ngemuva kokuthi amaseli we-HEK293 elashwe ngokugxilisa okuhlukile kwe-pepstatin A amahora angama-24, ukuvezwa kwe-LC3Ⅱ ne-p62 kwatholwa.Imiphumela yabonisa ukuthi i-pepstatin A ingathuthukisa kakhulu inkulumo ye-LC3Ⅱ ne-P62 (P<0.05) ngendlela encike kumthamo.I-20μ g/ml i-pepstatin A yayisetshenziselwa ukwelapha i-HEK293 ngezikhathi ezihlukene, futhi imiphumela yezikhawu zesikhathi ezihlukene ekuvezweni kwe-LC3II ne-p62 yabonwa.Imiphumela ibonise ukuthi i-pepstatin A ingakwazi ukulawula ukuvezwa kwe-LC3II ne-p62 ngendlela encike esikhathini.
Singumkhiqizi we-polypeptide e-China, esineminyaka eminingana yokuhlangenwe nakho okuvuthiwe ekukhiqizeni i-polypeptide.I-Hangzhou Taijia Biotech Co., Ltd. ingumkhiqizi wezinto ezingavuthiwe ze-polypeptide, enganikeza amashumi ezinkulungwane zezinto zokusetshenziswa ze-polypeptide futhi ingenziwa ngokwezifiso ngokwezidingo.Izinga lemikhiqizo ye-polypeptide lihle kakhulu, futhi ubumsulwa bungafinyelela ku-98%, okuye kwaqashelwa abasebenzisi emhlabeni wonke.Siyakwamukela ukuthi uxhumane nathi.
